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antibodies against runx2  (Proteintech)


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    Structured Review

    Proteintech antibodies against runx2
    Fstl1 enhances the osteogenic differentiation potential of MC3T3-E1 cells. (A) Representative alkaline-phosphatase (ALP) staining at 7 d. (B) Quantitative ALP activity. (C) Representative Alizarin Red S (ARS) staining at 14 d. (D) Quantification of ARS absorbance (OD 562 ). (E-H) Relative mRNA expression of <t>Runx2,</t> Sp7, Ocn, and Opn at 14 d determined by RT-qPCR. (I) Western-blot bands for RUNX2 and SP7. (J and K) Densitometric ratios of RUNX2/GAPDH and SP7/GAPDH. Sample sizes: con ( n = 3), OE-Fstl1 ( n = 3), and sh-Fstl1 ( n = 3). Data are presented as mean ± SD; inter-group comparisons were performed using 1-way ANOVA. * p < .05, ** p < .01, *** p < .001, **** p < .0001; ns, not significant.
    Antibodies Against Runx2, supplied by Proteintech, used in various techniques. Bioz Stars score: 94/100, based on 13 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/antibody+against+runx2/RUNX2+Fusion+Protein/pmc13050501-80-0-21
    Average 94 stars, based on 13 article reviews
    antibodies against runx2 - by Bioz Stars, 2026-11
    94/100 stars

    Images

    1) Product Images from "Follistatin-like protein 1 (FSTL1) modulates bone remodeling and attenuates bone loss in a mouse model of postmenopausal osteoporosis"

    Article Title: Follistatin-like protein 1 (FSTL1) modulates bone remodeling and attenuates bone loss in a mouse model of postmenopausal osteoporosis

    Journal: JBMR Plus

    doi: 10.1093/jbmrpl/ziag037

    Fstl1 enhances the osteogenic differentiation potential of MC3T3-E1 cells. (A) Representative alkaline-phosphatase (ALP) staining at 7 d. (B) Quantitative ALP activity. (C) Representative Alizarin Red S (ARS) staining at 14 d. (D) Quantification of ARS absorbance (OD 562 ). (E-H) Relative mRNA expression of Runx2, Sp7, Ocn, and Opn at 14 d determined by RT-qPCR. (I) Western-blot bands for RUNX2 and SP7. (J and K) Densitometric ratios of RUNX2/GAPDH and SP7/GAPDH. Sample sizes: con ( n = 3), OE-Fstl1 ( n = 3), and sh-Fstl1 ( n = 3). Data are presented as mean ± SD; inter-group comparisons were performed using 1-way ANOVA. * p < .05, ** p < .01, *** p < .001, **** p < .0001; ns, not significant.
    Figure Legend Snippet: Fstl1 enhances the osteogenic differentiation potential of MC3T3-E1 cells. (A) Representative alkaline-phosphatase (ALP) staining at 7 d. (B) Quantitative ALP activity. (C) Representative Alizarin Red S (ARS) staining at 14 d. (D) Quantification of ARS absorbance (OD 562 ). (E-H) Relative mRNA expression of Runx2, Sp7, Ocn, and Opn at 14 d determined by RT-qPCR. (I) Western-blot bands for RUNX2 and SP7. (J and K) Densitometric ratios of RUNX2/GAPDH and SP7/GAPDH. Sample sizes: con ( n = 3), OE-Fstl1 ( n = 3), and sh-Fstl1 ( n = 3). Data are presented as mean ± SD; inter-group comparisons were performed using 1-way ANOVA. * p < .05, ** p < .01, *** p < .001, **** p < .0001; ns, not significant.

    Techniques Used: Staining, Activity Assay, Expressing, Quantitative RT-PCR, Western Blot

    Related Articles

    Incubation:

    Article Title: An Injectable, Osteoconductive Gelatin-Enabled GelMA/HAp Hydrogel Scaffold for Minimally Invasive Bone Tissue Engineering
    Article Snippet: .. The fixed samples were permeabilized and blocked, followed by incubation with a primary antibody against Runx2 (Shanghai, China, Proteintech, 20700-1-AP) and then with the corresponding fluorophore-conjugated secondary antibody (USA, Thermo Fisher Scientific, A-11011). .. Subsequently, the cytoskeleton was stained with FITC-conjugated phalloidin (China, Proteintech, PF00001) to visualize F-actin, and the nuclei were counterstained with DAPI (China, Beyotime, C1005).

    Article Title: OsteoNet: A deep learning framework linking cellular morphology to molecular markers for quantifying osteogenic differentiation
    Article Snippet: Data were processed using FlowJo software. .. Cells cultured on sterile glass coverslips were fixed with 4 % paraformaldehyde for 15 min at room temperature and subsequently permeabilized with 0.1 % Triton X-100 for 10 min. After blocking non-specific binding sites with 5 % bovine serum albumin (BSA) for 30 min, the cells were incubated with a primary antibody against RUNX2 (Proteintech) or OCN (Proteintech) overnight at 4°C. .. Cells cultured on sterile glass coverslips were fixed with 4 % paraformaldehyde for 15 min at room temperature and subsequently permeabilized with 0.1 % Triton X-100 for 10 min. After blocking non-specific binding sites with 5 % bovine serum albumin (BSA) for 30 min, the cells were incubated with a primary antibody against RUNX2 (Proteintech) or OCN (Proteintech) overnight at 4°C.

    Cell Culture:

    Article Title: OsteoNet: A deep learning framework linking cellular morphology to molecular markers for quantifying osteogenic differentiation
    Article Snippet: Data were processed using FlowJo software. .. Cells cultured on sterile glass coverslips were fixed with 4 % paraformaldehyde for 15 min at room temperature and subsequently permeabilized with 0.1 % Triton X-100 for 10 min. After blocking non-specific binding sites with 5 % bovine serum albumin (BSA) for 30 min, the cells were incubated with a primary antibody against RUNX2 (Proteintech) or OCN (Proteintech) overnight at 4°C. .. Cells cultured on sterile glass coverslips were fixed with 4 % paraformaldehyde for 15 min at room temperature and subsequently permeabilized with 0.1 % Triton X-100 for 10 min. After blocking non-specific binding sites with 5 % bovine serum albumin (BSA) for 30 min, the cells were incubated with a primary antibody against RUNX2 (Proteintech) or OCN (Proteintech) overnight at 4°C.

    Sterility:

    Article Title: OsteoNet: A deep learning framework linking cellular morphology to molecular markers for quantifying osteogenic differentiation
    Article Snippet: Data were processed using FlowJo software. .. Cells cultured on sterile glass coverslips were fixed with 4 % paraformaldehyde for 15 min at room temperature and subsequently permeabilized with 0.1 % Triton X-100 for 10 min. After blocking non-specific binding sites with 5 % bovine serum albumin (BSA) for 30 min, the cells were incubated with a primary antibody against RUNX2 (Proteintech) or OCN (Proteintech) overnight at 4°C. .. Cells cultured on sterile glass coverslips were fixed with 4 % paraformaldehyde for 15 min at room temperature and subsequently permeabilized with 0.1 % Triton X-100 for 10 min. After blocking non-specific binding sites with 5 % bovine serum albumin (BSA) for 30 min, the cells were incubated with a primary antibody against RUNX2 (Proteintech) or OCN (Proteintech) overnight at 4°C.

    Blocking Assay:

    Article Title: OsteoNet: A deep learning framework linking cellular morphology to molecular markers for quantifying osteogenic differentiation
    Article Snippet: Data were processed using FlowJo software. .. Cells cultured on sterile glass coverslips were fixed with 4 % paraformaldehyde for 15 min at room temperature and subsequently permeabilized with 0.1 % Triton X-100 for 10 min. After blocking non-specific binding sites with 5 % bovine serum albumin (BSA) for 30 min, the cells were incubated with a primary antibody against RUNX2 (Proteintech) or OCN (Proteintech) overnight at 4°C. .. Cells cultured on sterile glass coverslips were fixed with 4 % paraformaldehyde for 15 min at room temperature and subsequently permeabilized with 0.1 % Triton X-100 for 10 min. After blocking non-specific binding sites with 5 % bovine serum albumin (BSA) for 30 min, the cells were incubated with a primary antibody against RUNX2 (Proteintech) or OCN (Proteintech) overnight at 4°C.

    Binding Assay:

    Article Title: OsteoNet: A deep learning framework linking cellular morphology to molecular markers for quantifying osteogenic differentiation
    Article Snippet: Data were processed using FlowJo software. .. Cells cultured on sterile glass coverslips were fixed with 4 % paraformaldehyde for 15 min at room temperature and subsequently permeabilized with 0.1 % Triton X-100 for 10 min. After blocking non-specific binding sites with 5 % bovine serum albumin (BSA) for 30 min, the cells were incubated with a primary antibody against RUNX2 (Proteintech) or OCN (Proteintech) overnight at 4°C. .. Cells cultured on sterile glass coverslips were fixed with 4 % paraformaldehyde for 15 min at room temperature and subsequently permeabilized with 0.1 % Triton X-100 for 10 min. After blocking non-specific binding sites with 5 % bovine serum albumin (BSA) for 30 min, the cells were incubated with a primary antibody against RUNX2 (Proteintech) or OCN (Proteintech) overnight at 4°C.



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    MedChemExpress antibodies against runx2
    Osteogenic differentiation of hPDLSCs induced by PDA@Hb/NOCG. ( A ) Representative ALP staining results of hPDLSCs after 7 days of osteogenic differentiation. ( B ) Alizarin Red S staining of hPDLSCs after 21 days of osteogenic induction. ( C ) Quantitative analysis of ALP staining in hPDLSCs. ( D ) Quantification of Alizarin Red S staining in hPDLSCs. ( E-K ) Relative mRNA expression levels of osteogenesis-related genes in hPDLSCs, including ALP, BMP2, COL1A1, OPG, <t>RUNX2,</t> OPN, and OCN, normalized to GAPDH. ( L-O ) Protein expression levels of osteogenesis-related markers in hPDLSCs, including ALP, RUNX2, and β-catenin, and their corresponding quantitative analysis. ( P ) Protein expression levels of osteogenesis-related markers, including ALP, RUNX2, and β-catenin, in hPDLSCs under different treatment conditions with or without DKK1. ( Q ) Corresponding quantitative analysis of ALP, RUNX2, and β-catenin protein expression in each group
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    Osteogenic differentiation of hPDLSCs induced by PDA@Hb/NOCG. ( A ) Representative ALP staining results of hPDLSCs after 7 days of osteogenic differentiation. ( B ) Alizarin Red S staining of hPDLSCs after 21 days of osteogenic induction. ( C ) Quantitative analysis of ALP staining in hPDLSCs. ( D ) Quantification of Alizarin Red S staining in hPDLSCs. ( E-K ) Relative mRNA expression levels of osteogenesis-related genes in hPDLSCs, including ALP, BMP2, COL1A1, OPG, <t>RUNX2,</t> OPN, and OCN, normalized to GAPDH. ( L-O ) Protein expression levels of osteogenesis-related markers in hPDLSCs, including ALP, RUNX2, and β-catenin, and their corresponding quantitative analysis. ( P ) Protein expression levels of osteogenesis-related markers, including ALP, RUNX2, and β-catenin, in hPDLSCs under different treatment conditions with or without DKK1. ( Q ) Corresponding quantitative analysis of ALP, RUNX2, and β-catenin protein expression in each group
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    Osteogenic differentiation-promoting effect of DBM-DMSN@TPTD composite scaffold on BMSCs. ( A ) ALP staining of BMSCs after 7 days of culture. ( B ) Immunofluorescence staining of <t>RUNX2</t> in BMSCs. ( C ) Immunofluorescence staining of OCN in BMSCs. ( D ) Quantitative analysis of RUNX2 mean fluorescence intensity. ( E ) Quantitative analysis of OCN mean fluorescence intensity. Data are presented as mean ± SD ( n = 3). Comparisons among multiple groups were performed using one-way ANOVA followed by Tukey’s multiple comparisons post hoc test. ns, no significant difference; * p < 0.05; ** p < 0.01; *** p < 0.001.
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    Proteintech antibodies against runx2
    Fstl1 enhances the osteogenic differentiation potential of MC3T3-E1 cells. (A) Representative alkaline-phosphatase (ALP) staining at 7 d. (B) Quantitative ALP activity. (C) Representative Alizarin Red S (ARS) staining at 14 d. (D) Quantification of ARS absorbance (OD 562 ). (E-H) Relative mRNA expression of <t>Runx2,</t> Sp7, Ocn, and Opn at 14 d determined by RT-qPCR. (I) Western-blot bands for RUNX2 and SP7. (J and K) Densitometric ratios of RUNX2/GAPDH and SP7/GAPDH. Sample sizes: con ( n = 3), OE-Fstl1 ( n = 3), and sh-Fstl1 ( n = 3). Data are presented as mean ± SD; inter-group comparisons were performed using 1-way ANOVA. * p < .05, ** p < .01, *** p < .001, **** p < .0001; ns, not significant.
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    Cell Signaling Technology Inc primary antibodies against runx2
    Fstl1 enhances the osteogenic differentiation potential of MC3T3-E1 cells. (A) Representative alkaline-phosphatase (ALP) staining at 7 d. (B) Quantitative ALP activity. (C) Representative Alizarin Red S (ARS) staining at 14 d. (D) Quantification of ARS absorbance (OD 562 ). (E-H) Relative mRNA expression of <t>Runx2,</t> Sp7, Ocn, and Opn at 14 d determined by RT-qPCR. (I) Western-blot bands for RUNX2 and SP7. (J and K) Densitometric ratios of RUNX2/GAPDH and SP7/GAPDH. Sample sizes: con ( n = 3), OE-Fstl1 ( n = 3), and sh-Fstl1 ( n = 3). Data are presented as mean ± SD; inter-group comparisons were performed using 1-way ANOVA. * p < .05, ** p < .01, *** p < .001, **** p < .0001; ns, not significant.
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    Fstl1 enhances the osteogenic differentiation potential of MC3T3-E1 cells. (A) Representative alkaline-phosphatase (ALP) staining at 7 d. (B) Quantitative ALP activity. (C) Representative Alizarin Red S (ARS) staining at 14 d. (D) Quantification of ARS absorbance (OD 562 ). (E-H) Relative mRNA expression of <t>Runx2,</t> Sp7, Ocn, and Opn at 14 d determined by RT-qPCR. (I) Western-blot bands for RUNX2 and SP7. (J and K) Densitometric ratios of RUNX2/GAPDH and SP7/GAPDH. Sample sizes: con ( n = 3), OE-Fstl1 ( n = 3), and sh-Fstl1 ( n = 3). Data are presented as mean ± SD; inter-group comparisons were performed using 1-way ANOVA. * p < .05, ** p < .01, *** p < .001, **** p < .0001; ns, not significant.
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    Image Search Results


    Osteogenic differentiation of hPDLSCs induced by PDA@Hb/NOCG. ( A ) Representative ALP staining results of hPDLSCs after 7 days of osteogenic differentiation. ( B ) Alizarin Red S staining of hPDLSCs after 21 days of osteogenic induction. ( C ) Quantitative analysis of ALP staining in hPDLSCs. ( D ) Quantification of Alizarin Red S staining in hPDLSCs. ( E-K ) Relative mRNA expression levels of osteogenesis-related genes in hPDLSCs, including ALP, BMP2, COL1A1, OPG, RUNX2, OPN, and OCN, normalized to GAPDH. ( L-O ) Protein expression levels of osteogenesis-related markers in hPDLSCs, including ALP, RUNX2, and β-catenin, and their corresponding quantitative analysis. ( P ) Protein expression levels of osteogenesis-related markers, including ALP, RUNX2, and β-catenin, in hPDLSCs under different treatment conditions with or without DKK1. ( Q ) Corresponding quantitative analysis of ALP, RUNX2, and β-catenin protein expression in each group

    Journal: Journal of Nanobiotechnology

    Article Title: Nanoparticle-integrated smart hydrogel with H 2 S-triggered antibacterial escalation and sustained oxygen release for periodontal regeneration

    doi: 10.1186/s12951-026-04535-7

    Figure Lengend Snippet: Osteogenic differentiation of hPDLSCs induced by PDA@Hb/NOCG. ( A ) Representative ALP staining results of hPDLSCs after 7 days of osteogenic differentiation. ( B ) Alizarin Red S staining of hPDLSCs after 21 days of osteogenic induction. ( C ) Quantitative analysis of ALP staining in hPDLSCs. ( D ) Quantification of Alizarin Red S staining in hPDLSCs. ( E-K ) Relative mRNA expression levels of osteogenesis-related genes in hPDLSCs, including ALP, BMP2, COL1A1, OPG, RUNX2, OPN, and OCN, normalized to GAPDH. ( L-O ) Protein expression levels of osteogenesis-related markers in hPDLSCs, including ALP, RUNX2, and β-catenin, and their corresponding quantitative analysis. ( P ) Protein expression levels of osteogenesis-related markers, including ALP, RUNX2, and β-catenin, in hPDLSCs under different treatment conditions with or without DKK1. ( Q ) Corresponding quantitative analysis of ALP, RUNX2, and β-catenin protein expression in each group

    Article Snippet: Primary antibodies against ALP (rabbit polyclonal IgG, Cat. No. 18507-1-AP), NF-κB p65 (rabbit polyclonal IgG, Cat. No. 10745-1-AP), and β-catenin (rabbit polyclonal IgG, Cat. No. 17565-1-AP) were purchased from Proteintech (Wuhan, China), while the antibodies against RUNX2 (rabbit polyclonal IgG, Cat. No. HY- P80316 ) and Hb (polyclonal antibody, Cat. No. PAB409Bo01) were obtained from MedChemExpress (MCE, Shanghai, China) and Cloud-Clone Corp. (Wuhan, China), respectively.

    Techniques: Staining, Expressing

    Osteogenic differentiation-promoting effect of DBM-DMSN@TPTD composite scaffold on BMSCs. ( A ) ALP staining of BMSCs after 7 days of culture. ( B ) Immunofluorescence staining of RUNX2 in BMSCs. ( C ) Immunofluorescence staining of OCN in BMSCs. ( D ) Quantitative analysis of RUNX2 mean fluorescence intensity. ( E ) Quantitative analysis of OCN mean fluorescence intensity. Data are presented as mean ± SD ( n = 3). Comparisons among multiple groups were performed using one-way ANOVA followed by Tukey’s multiple comparisons post hoc test. ns, no significant difference; * p < 0.05; ** p < 0.01; *** p < 0.001.

    Journal: Pharmaceutics

    Article Title: Dendritic Mesoporous Silica-Modified Decellularized Bone Matrix Scaffold for Sustained Teriparatide Delivery in Bone Defect Repair: Characterization, Drug Release, and In Vitro Biological Performance

    doi: 10.3390/pharmaceutics18091067

    Figure Lengend Snippet: Osteogenic differentiation-promoting effect of DBM-DMSN@TPTD composite scaffold on BMSCs. ( A ) ALP staining of BMSCs after 7 days of culture. ( B ) Immunofluorescence staining of RUNX2 in BMSCs. ( C ) Immunofluorescence staining of OCN in BMSCs. ( D ) Quantitative analysis of RUNX2 mean fluorescence intensity. ( E ) Quantitative analysis of OCN mean fluorescence intensity. Data are presented as mean ± SD ( n = 3). Comparisons among multiple groups were performed using one-way ANOVA followed by Tukey’s multiple comparisons post hoc test. ns, no significant difference; * p < 0.05; ** p < 0.01; *** p < 0.001.

    Article Snippet: Subsequently, the cells were blocked with 5% bovine serum albumin (BSA, Sigma-Aldrich, St. Louis, MO, USA) at 37 °C for 30 min. Primary antibodies against RUNX2 (ABclonal Technology Co., Ltd., Wuhan, Hubei, China) and OCN (ABclonal Technology Co., Ltd., Wuhan, Hubei, China) were added and incubated overnight at 4 °C in a humidified chamber.

    Techniques: Staining, Immunofluorescence, Analysis, Fluorescence

    Fstl1 enhances the osteogenic differentiation potential of MC3T3-E1 cells. (A) Representative alkaline-phosphatase (ALP) staining at 7 d. (B) Quantitative ALP activity. (C) Representative Alizarin Red S (ARS) staining at 14 d. (D) Quantification of ARS absorbance (OD 562 ). (E-H) Relative mRNA expression of Runx2, Sp7, Ocn, and Opn at 14 d determined by RT-qPCR. (I) Western-blot bands for RUNX2 and SP7. (J and K) Densitometric ratios of RUNX2/GAPDH and SP7/GAPDH. Sample sizes: con ( n = 3), OE-Fstl1 ( n = 3), and sh-Fstl1 ( n = 3). Data are presented as mean ± SD; inter-group comparisons were performed using 1-way ANOVA. * p < .05, ** p < .01, *** p < .001, **** p < .0001; ns, not significant.

    Journal: JBMR Plus

    Article Title: Follistatin-like protein 1 (FSTL1) modulates bone remodeling and attenuates bone loss in a mouse model of postmenopausal osteoporosis

    doi: 10.1093/jbmrpl/ziag037

    Figure Lengend Snippet: Fstl1 enhances the osteogenic differentiation potential of MC3T3-E1 cells. (A) Representative alkaline-phosphatase (ALP) staining at 7 d. (B) Quantitative ALP activity. (C) Representative Alizarin Red S (ARS) staining at 14 d. (D) Quantification of ARS absorbance (OD 562 ). (E-H) Relative mRNA expression of Runx2, Sp7, Ocn, and Opn at 14 d determined by RT-qPCR. (I) Western-blot bands for RUNX2 and SP7. (J and K) Densitometric ratios of RUNX2/GAPDH and SP7/GAPDH. Sample sizes: con ( n = 3), OE-Fstl1 ( n = 3), and sh-Fstl1 ( n = 3). Data are presented as mean ± SD; inter-group comparisons were performed using 1-way ANOVA. * p < .05, ** p < .01, *** p < .001, **** p < .0001; ns, not significant.

    Article Snippet: Antibodies against RUNX2, SP7, NFATC1, and CTSK were purchased from Abcam, while the anti-GAPDH antibody and anti-FSTL1 antibody were obtained from Proteintech.

    Techniques: Staining, Activity Assay, Expressing, Quantitative RT-PCR, Western Blot